陕西师范大学学报（自然科学版）2013，Vol.41Issue(2)：76-81,6.
两种苦瓜多糖对人白血病细胞体外增殖的影响
The inhibition effect of two different momordica charantia polysaccharides on the proliferation of human leukemia cell in vitro
摘要
Abstract
The crude polysaccharides were extracted from momordica charantia using hot water. Then they were isolated and purified by DEAE-52-cellulose anion exchange chromatography. After eluted by double distilled water and 0. 1 mol/L NaCl respectively, the W-MCP and S-MCP were obtained. The in vitro inhibition activity of W-MCP and S-MCP on the proliferation of human leukemia cell line K562 were measured by using MTT assay. The in vitro antioxidant activity including O2-·and DPPH free radical scavenging effect and lipid peroxidation inhibition effect of W-MCP and S-MCP were determined. Our results showed that the inhibition rate on the proliferation of human leukemia cell line K562 of W-MCP and S-MCP increased with time. The inhibition rate tended to be stable at 48h, and the largest inhibition rate of W-MCP and S-MCP reached 30. 46% and 28. 61% at the concentration of 400μg/mL at 72h. Both W-MCP and S-MCP have high scavenging rate on the O2- · , reaching 58. 52% and 44. 71%. The DPPH scavenging rate of W-MCP and S-MCP decreased with the concentration, and the highest scavenging rate were 19.01% and 30. 11%, respectively. Lipid peroxidation inhibition effect of W-MCP and S-MCP was low. Therefore, balsam pear polysaccharides had certain antioxidant activity and inhibition effect on cancer cell proliferation.关键词
水洗苦瓜多糖/盐洗苦瓜多糖/抗肿瘤/抗氧化Key words
water elution balsam pear polysaccharide/ salt elution balsam pear polysaccharide/ antitumor/ antioxidant分类
医药卫生引用本文复制引用
李哲涛,张静,谢佳,孙润广..两种苦瓜多糖对人白血病细胞体外增殖的影响[J].陕西师范大学学报（自然科学版）,2013,41(2):76-81,6.基金项目
国家自然科学基金资助项目(10874108) （10874108）
陕西省自然科学基础研究计划项目(SJ08A16). （SJ08A16）
