实用医学杂志2013，Vol.29Issue(5)：697-700,4.DOI:10.3969/j.issn.1006-5725.2013.05.007
纳米氧化铁介导的mdr1反义寡核苷酸对人肠癌细胞的靶向投递作用
Targeting Delivery Effect of Magnetic Iron Oxide Nanoparticles-mediated mdr1 Antisense Oligonucleotide on Human Colorectal Carcinoma cell
摘要
Abstract
Objective To study the Magnetic Iron Oxide Nanoparticles as a carrier-mediated MDR1 asODN on human colorectal Carcinoma cells, and evaluate the targeting delivery effect. Method LS174T、HCT-8/ VCR cultured with PLL-DCIONP-asODN-FAM、 asODN-FAM in vitro were observed under fluorescence microscope. LS174T、 HCT-8/VCR cultured with PLL-DCIONP-asODN、 PLL-DCIONP in vitro were observed by Perle's blue staining. In addition, the change of magnetic singal intensity was measured by 1.5T MRI. Results The fluorescence intensity of transfected PLL-DCIONP-asODN-FAM in LS174T, HCT-8/VCR cells was significantly higher than asODN-FAM transfection group. The blue iron particles of transfected PLL-DCIONP-asODN in LS174T, HCT-8/VCR cells were significantly more than PLL-DCIONP transfection group. After transfaction by PLL-DCIONP-asODN the cells spin-spin relaxitions were shortened gradually compared with increasing labeled cells and showed a trend to attenuation. Conclusions PLL-DCIONP-asODN has high targeting delivery effect on HCT-8/VCR.关键词
氧化铁磁性纳米颗粒/反义寡聚核苷酸/肠癌Key words
Magnetic iron oxide nanoparticles/ Antisense oligonucleotide/ colorectal Carcinoma引用本文复制引用
王金玉,武兆忠,陈江林,李娜玲,蓝文珍..纳米氧化铁介导的mdr1反义寡核苷酸对人肠癌细胞的靶向投递作用[J].实用医学杂志,2013,29(5):697-700,4.基金项目
国家自然科学基金面上项目(编号:30470516) （编号:30470516）
