天然产物研究与开发2013，Vol.25Issue(2)：211-216,6.
Folin-Ciocalteu分光光度法测定夏枯草总酚含量的研究
Spectrophotometric Determination of the Total Phenolic Content in Prunella vulgaris L.by Folin-Ciocalteu Reagent
摘要
Abstract
With gallic acid as the standard,the content of the total phenols in Prunella vulgaris L.was determined by the spectrophotometric method using Folin-Ciocalteu reagent.The optimized reaction conditions were ambient temperature,0.5 mL of the Folin-Ciocalteu reagent,1.7 mL of 20% Na2CO3 with 60 min reaction.Two absorption peaks at wavelengths 660 nm and 760 nm were investigated in this study.Good linearity in the concentration range from 0 to 10.3 μg/mL was achieved for both wavelengths.The regression equations were found to be:at 660 nm,Y =94.542X-0.0067,R2 =0.9999 and the recovery of total polyphenols was 101.7 ～ 105.0% ; at 760 nm,Y =101.13X-0.0191,R2 =0.9990and the recovery of total polyphenols was 105.1 ～ 107.6%.This method demonstrated to have had good stability,accuracy and repeatability.The work presented in this study provided a reference for quantitative analysis of total phenols of Prunella vulgaris L.关键词
夏枯草/Folin-Ciocalteu试剂/分光光度法/总酚Key words
Prunella vulgaris L./ Folin-Ciocalteu reagent /spectrophotometry /total phenols分类
医药卫生引用本文复制引用
刘子衿,李倩,张枫..Folin-Ciocalteu分光光度法测定夏枯草总酚含量的研究[J].天然产物研究与开发,2013,25(2):211-216,6.基金项目
This work was supported by the Beijing Municipal Education Commission for the laboratory construction(01422600012013-001549) and talent training mode innovation test site(01422600012013-001538). （01422600012013-001549）
