武警医学2013，Vol.24Issue(4)：310-312,3.
洛菲不动杆菌株多药耐药NDM-1基因的鉴定
Identification on Multidrug resistance Gene of New Delhi metallo-beta-lactamase-1 in Acinetobacter lwoffii
摘要
Abstract
Objective To detect and identify multidrug resistance gene of new delhi metallo - beta - lactamase (NDM) 1 in a Acinetobacter lwoffii isolate. Methods Tigecycline MICs were used to screen the multidrug isolate while PCR were used to detect the expression of metallo - β - lactamases among the isolates. Species identification and phenotype analysis were carried out by PCR sequencing, Imipenem and EDTA double disc synergy test, respectively. Genetic location of NDM - 1 was determined by Conjugation experiments and whole genome sequencing. Results One strain of A. lwoffii with positive expression of NDM - 1 was isolated from 458 carbapenem - non - susceptible cases, which without VIM and IMP genes positive expression concomitantly. This strain with expression of NDM - 1 gene resist to all β - lactam antibiotics while retaining the susceptibility to amikacin, colistin and tigecycline. Sequencing showed 100% identities with previously reported genes, and whole genome sequencing conformed NDM - 1 gene located on a 48. 9 kb plasmid. Conclusions NDM - 1, as a naive mechanism, involved in resistance to carbopenems. The clinical physicians should pay more attention on impact of multidrug resistance of Acinetobacter lwoffii due to the expression of NDM - 1.关键词
新德里金属β-内酰胺酶/洛菲不动杆菌/多药耐药基因Key words
new delhi metallo - β - lactamase/ acinetobacter lwoffii/ multidrug resistance gene分类
医药卫生引用本文复制引用
张薇,梁慧,刘丽萍,彭国钧,胡红焱..洛菲不动杆菌株多药耐药NDM-1基因的鉴定[J].武警医学,2013,24(4):310-312,3.基金项目
武警总医院苗圃基金 (WZ2010049) （WZ2010049）
