新疆农业科学2013，Vol.50Issue(2)：357-364,8.DOI:10.6048/j.issn.1001-4330.2013.02.022
新疆南疆猪繁殖与呼吸综合征病毒ORF5基因克隆与序列分析
The Cloning and the Sequence Analysis of Southern Xinjiang Strain ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
[ Objective ] This project aims to grasp the characteristics of pandemic strain of the Southern Xinjiang PRRSV and provide a theoretical basis for the effective prevention and control of the virus. [ Method] In this study, eight complete sequences of 0RF5 genes of the South Xinjiang PRRSV were obtained by cloning and sequence analysis. [Result] The gene length of eight complete sequences of ORF5 genes of the Southern Xinjiang PRRSV were all 603 bp, nucleotide homology between them was 87.4% to 100.0% ; The nucleotide homology of ORF5 genes were 60. 9% to 63. 5% , 88. 4% to 89. 9% , 88. 9% to 94. 7% , and 87.4% to 98. 7% , respectively, compared with LV of Europe type representative strain, ATCC VR -2332 of American type representative strain, CH - la of China type representative strain, and JXA1 of the HP -PRRS 9China type representative strains. And compared with attenuated vaccine strains used wildly in China pig farms, their nucleotide homology was 87. 4% to 94.7% . The amino acids homology of ORF5 genes compared with the American type representative strain and Europe type strain was 84. 5% to 98. 0% and 50. 0% to 59. 5% . [ Conclusion] The five ORF5 genes of the Southern Xinjiang PRRSV all belong to the American type subgroup Ⅱ strains. The molecular epidemiology investigation result of the Southern Xinjiang PRRSV has laid the foundation for the further adjustment and guidance in the choice of vaccine for Southern Xinjiang PRRS.关键词
新疆南疆/猪繁殖与呼吸综合征/ORF5基因/序列分析Key words
Southern Xinjiang/ porcine reproductive and respiratory syndrome (PRRS)/ ORF5 gene/ sequence analysis分类
农业科技引用本文复制引用
王旭哲,刘永宏,赵丽,焦海宏,李龙凯,胡天成,段文婷,赵明明..新疆南疆猪繁殖与呼吸综合征病毒ORF5基因克隆与序列分析[J].新疆农业科学,2013,50(2):357-364,8.基金项目
国家大学生创新创业训练计划项目(TDGCX201231) （TDGCX201231）
新疆生产建设兵团博士资金项目(2011BB022) （2011BB022）
