新医学2013，Vol.44Issue(3)：206-210,5.DOI:10.3969/g.issn.0253-9802.2013.03.016
神经营养因子-3联合神经干细胞移植治疗缺血缺氧性脑损伤乳鼠的病理实验研究
Pathology of Neurotrophic factor-3 combined Neural stem cell transplantation in the treatment of ischemic brain damage suckling rats
摘要
Abstract
Objective: To determine the effect of neurotrophin-3 combined with neural stem cell graft in neu rological function recovery of hypoxic-ischemic brain injury in suckling rats. Methods:Rat fetal cerebral cortical tissue NSCs was cultured in vitro and passaged, then cultured neural stem cells were suspended. 60 suckling rats were be divided into A, B, C and D, four groups by radom number; normal group (A) , hypoxia-ischemia model group (B) , the simple neural stem cell transplantation group (C) , NT-3 joint neural stem cell transplantation group (D). The classic ischemia and hypoxia model has been used for the balace beam test 3 days later; group A weren't given any intervention, group B were given saline on the third day after surgery, group C were transplanted by NSCs and the mice of group D were transplanted by NSC together with NT-3. 4 weeks after transplantation, the brains were removed for gross specimens, HE , immunohistochemical staining pathological comparison. Results:① The bulk multicelluar spheroids in cortex graft in vitro cultured rats' tiss were neural stemcell according to positive Nestin staining. ②Compared to group B, the blood supply of grop C and D increased, and the degree of the brain tiss atrophying and collapsing decreased. ③Besause of HE staining, the cells around lateral ventricle in group B were swelling, degeneration, necrosis, arranged disorder, local cystic changes and inflammatory cells accumula tion. Compared to structural damage, the damage degree of normal tissue in group C a improved, which was better in group D. ④In immune past, the neuroglia positive cells in group B were more than that in group A, and oligo dendroglia cells in group B were less. Compared to group B, the neuroglia cells in group C were less and oligoden droglia cells in group C were more. The situation of group D was the best. Conclusion: NT-3 can promote the re covery of neurological function of hypoxic-ischemic brain injury patients by promoting neural stem cell proliferation and differentiation关键词
缺血缺氧性脑损伤/神经干细胞/神经营养因子-3/细胞移植/神经功能恢复Key words
Hypoxic-ischemic brain injury/Neural stem cell/Neural trophic -3/Transplantation/Functional recovery引用本文复制引用
李凯,冯冠军,胡艳荣,杨小朋,陈刚,刘伟,张艳君..神经营养因子-3联合神经干细胞移植治疗缺血缺氧性脑损伤乳鼠的病理实验研究[J].新医学,2013,44(3):206-210,5.基金项目
国家自然科学基金(30960393 （）
81160155) （）
