医药导报2013，Vol.32Issue(3)：356-359,4.DOI:10.3870/yydb.2013.03.028
高效液相色谱法测定鳖甲煎丸中芍药苷和黄芩苷的含量
Determination of Paeoniflorin and Baicalin in Biejiajian Pill by HPLC
摘要
Abstract
Objective An HPLC method was established for the determination of paeoniflorin and baicalin in biejiajian pill. Methods Paeoniflorin was separated on a C18 column with gradient elution using the mobile phase of acetonitrile 0.05 mol· L-1 potassium dihydrogen phosphate solution,and detected at the wavelength of 230 nm. Baicalin was separated on the same column with methanol-water-phosphoric acid (42 :58 : 0. 5) as mobile phase, and detected at the wavelength of 280 nm. Results The calibration curves were linear in the ranges of 0. 076 6-1.532 8 μg and 0.061 4-0. 770 4 μg,the average recovery was 98.0% and 96. 5% ( n = 6) , with RSDs of 0. 7% and 1.1%, respectively. Conclusion Two analytical methods were established to determine the two active ingredients for quality control of the biejiajian Pill.关键词
鳖甲煎丸/芍药苷/黄芩苷/色谱法,高效液相Key words
Biejiajian pill/ Paeoniflorin/ Baicalin/ HPLC分类
医药卫生引用本文复制引用
张庆,马骏,辛俐华..高效液相色谱法测定鳖甲煎丸中芍药苷和黄芩苷的含量[J].医药导报,2013,32(3):356-359,4.
