医药导报2013，Vol.32Issue(4)：432-434,3.DOI:10.3870/yydb.2013.04.005
反相高效液相色谱法测定人血清齐拉西酮浓度
Determination of Ziprasidone in Human Serum by RP-HPLC
摘要
Abstract
Objective To develop a high performance liquid chromatography ( HPLC) method for the determination of ziprasidone in human serum. Methods The serum samples were extracted with ether and separated on an Zorbax Eclipse XDB C18 column using methanol-0.03 mol · L-1 ammonium acetate (70 : 30) as the mobile phase. The flow rate was 0.8 mL · min-1. The fractions were detected by diode array detector ( DAD) with wavelength at 254 nm. Results The linear range of ziprasidone was 10-1 000 ng · mL-1 . The correlation coefficient was 0. 991 7 and the limit of quantitation ( LOQ) was 10 ng · mL-1. The matrix effect was from 92. 1% to 106. 7% . The extraction recovery was in the range of 72. 3% - 80.4% (n = 5). The intra-day and inter-day precision (RSD) were 4.7%-6.4% (n = 5) and 8.9%-12.1% (n = 5), respectively. Conclusion The HPLC method is simple, sensitive and accurate. It is suitable for the determination of ziprasidone in human serum.关键词
齐拉西酮/色谱法,高效液相/血药浓度Key words
Ziprasidone/ HPLC/ Drug serum concentration分类
医药卫生引用本文复制引用
王泽民..反相高效液相色谱法测定人血清齐拉西酮浓度[J].医药导报,2013,32(4):432-434,3.基金项目
杭州市科技发展计划项目(20090833B) （20090833B）
杭州市卫生科技项目(项目编号:2010B023) （项目编号:2010B023）
