医药导报2013，Vol.32Issue(4)：442-445,4.DOI:10.3870/yydb.2013.04.008
Hint2促进乳腺癌细胞株MCF7对紫杉醇注射液的敏感性
Over-expression of Hint2 Sensitizes Breast Cancer Cell MCF7 to Taxol
摘要
Abstract
Objective To evaluate the effect of over-expression of Hint2 on sensitivity of breast cancer cell MCF7 to taxol . Methods The expression vector of pCMV-HA-Hint2 was constructed and transfected into MCF7 cells and the protein expression of Hint2 was detected by immunofluorescence and Western blotting. The changes of sensitivity to taxol after transfection were examined by MTT and flow cytometry respectively. Results In MCF7 cells, the protein levels of Hint2 were significantly increased after transfection, After exposed to 5 μmol · L-1 taxol for 24 h, cell viability of Hint2 over-expression group was 33. 78% , compared to 44. 12% in control group. Conclusion Over-expression of Hint2 can enhance the sensitivity of MCF7 to taxol.关键词
紫杉醇注射液/三联组氨酸核苷酸结合蛋白2/乳腺癌/MCF7Key words
Taxol/ Hint2/ Breast cancer/ MCF7分类
医药卫生引用本文复制引用
蔡少鑫,陈成,杨熹,邓豫,李兆明,李小兰,胡俊波..Hint2促进乳腺癌细胞株MCF7对紫杉醇注射液的敏感性[J].医药导报,2013,32(4):442-445,4.基金项目
国家973计划资助项目(2009CB521802) （2009CB521802）
国家自然科学基金资助项目(30872472,30973496,30800569). （30872472,30973496,30800569）
