应用化学2013，Vol.30Issue(3)：329-334,6.DOI:10.3724/SP.J.1095.2013.20174
多元曲线分辨-交替最小二乘法应用于延胡索药材指纹图谱中重叠峰分辨及延胡索乙素的含量测定
The Applications of Multivariate Curve Resolution-alternating Least Squares in Resolving Overlapped Spectra of Corydalis yanhusuo Fingerprint and the Content Detection of Tetrahydropalmatine
摘要
Abstract
Spectral correlative chromatography was used to identify the peak of tetrahydropalmatine in Corydalis yanhusuo fingerprint. Moreover, multivariate curve resolution-alternating least squares(MCR-ALS) was used to resolve the overlapping peaks of tetrahydropalmatine and restore its true peak shape. More than 99. 9% spectrum could be recovered and accurately quantitative results were obtained. The method can be used as an auxiliary fingerprinting method to provide a reliable basis for the authenticity and quality assessment of Chinese medicines. The effect of the herbs-cooking on the content of tetrahydropalmatine was also discussed.关键词
延胡索药材/中药指纹图谱/光谱相关色谱/多元曲线分辨-交替最小二乘法Key words
Corydalis yanhusuo, fingerprint of traditional Chinese medicine fingerprint, spectral correlative chromatography,分类
化学化工引用本文复制引用
贾泽慧,王春涛,李华..多元曲线分辨-交替最小二乘法应用于延胡索药材指纹图谱中重叠峰分辨及延胡索乙素的含量测定[J].应用化学,2013,30(3):329-334,6.基金项目
国家自然科学基金(20975081)、山西省自然科学基金(2011011007-2)资助项目 （20975081）
