中国骨伤2013，Vol.26Issue(3)：227-231,5.DOI:10.3969/j.issn.1003-0034.2013.03.014
骨细胞分离培养及其与成骨细胞鉴别比较的实验研究
Experimental study on the isolated culture of osteocytes and identification of osteoblasts in rats
摘要
Abstract
Objective:To establish a more stable method to isolate osteocytes in vitro,and then to find the differences with osteoblast biological characteristics. Methods: Osteocytes and osteoblasts were isolated from the bone tissue of 3-day-old rats using sequential collagenase digestion. The cells were identified through cell morphology after 24 hours later. Alkaline phos-phatase (ALP) kit was used to stain the first generation cells by Kaplow-way ,the bone gla protein (BGP) of the cells were stained by immunocytochemitry. Measured ALP and computed its activity. Results: Osteocytes and osteoblasts showed obviously differences in cell morphology. Osteocytes were star-shaped or dendrite-shaped within more dendrites,while osteoblasts were spindle-shaped with short dendrites. Osteocytes were negative for ALP,but osteoblasts were positive; Osteocytes were more positive for BGP, and osteoblasts were less positive. The secretion of ALP in osteocytes was lower than that of osteoblasts. Conclusion: Osteocytes can be isolated and cultured in vitro. These characteristics of osteocytes are apparently difference with those of osteoblasts.关键词
骨细胞/成骨细胞/细胞分离Key words
Osteocytes/ Osteoblasts/ Cell separation引用本文复制引用
汤小康,程婉,许兵,应航,童培建,肖鲁伟..骨细胞分离培养及其与成骨细胞鉴别比较的实验研究[J].中国骨伤,2013,26(3):227-231,5.基金项目
浙江省自然基金(编号:Y2110928) （编号:Y2110928）
浙江省中医药科学研究基金计划(编号:2010ZA020) （编号:2010ZA020）
