中国临床药理学与治疗学2013，Vol.18Issue(1)：24-29,6.
槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响
Effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells and the expression level of carcinoembryonic antigen
安昌勇 1谢刚 1汤为学 1杨小丁 1张才全1
作者信息
摘要
Abstract
AIM: To investigate the effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells as well as the expression level of carcinoembryonic antigen. METHODS: LOVO cells were treated with Quercetin at various concentrations,then determined for prolifer ation level by MTT assay, for cell adhesion and invasion of LOVO cell by tumor cell adhere to the vascular endothelial cell assay and transwell chamber invasion assay, and for the expression level of CEA by Immunocellulerchemistry (ICC), Western Blot and RT-PCR. RESULTS: Significantly, Quercetin showed dose-dependent inhibitory effect on proliferation of LOVO cells
with an IC50 of about 40 μmol/L, also improved the ability of LOVO cells adhere to the vascular endothelial cells and reduced the invasion ability of LOVO cells. Meanwhile, Quercetin signifi cantly inhibited the protein and mRNA expres sion levels of CEA in LOVO cells. CONCLU SION: Quercetin has inhibition effect on the proliferation invasion ability of LOVO cells,also inhibit the protein and mRNA expression levels of CEA in LOVO cells.关键词
槲皮素/结肠癌/增殖/侵袭/癌胚抗原Key words
Quercetin/Colorectal cancer/Proliferation/Invasion/CEA分类
医药卫生引用本文复制引用
安昌勇,谢刚,汤为学,杨小丁,张才全..槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响[J].中国临床药理学与治疗学,2013,18(1):24-29,6.
