中国美容医学2013，Vol.22Issue(2)：263-266,4.
辛伐他汀对人牙髓干细胞增殖和分化的影响
Effects of simvastatin on the proliferation and osteoblastic differentiation of human dental pulp stem cells
摘要
Abstract
Objective To study the effects of simvastatin on the proliferation and osteoblastic differentiation of human dental pulp stem cells (DPSCs). Methods The third passage human DPSCs were cultured in conditioned mineralization medium with different concentrations of simvastatin. Proliferation ability was detected by MTT method.Alkaline phosphatase activity was measured with ALP kit. Results Proliferation of human DPSCs was suppressed in all treatment groups with different concentrations of simvastatin(10-5mol/L,10-6mol/L,1×10-7mol/L,1×10-8mol/L). The maximum effect on the proliferation of DPSCs was observed at the concentration of 1×10~5mol/L.Osteoblastic differentiation of human DPSCs was increased in treatment group with different concentrations of simvastatin(10-6mol/L, 1 ×10 -7mol/L,1 ×10-8mol/L). The maximum effect on the activity of ALP was observed at the concentration of 1×10-7mol/L. Conclusion Proliferation of human DPSCs was suppressed by simvastatin.Optimal concentration of simvastatin can improve the osteoblastic differentiation of human DPSCs.关键词
辛伐他汀/牙髓干细胞/增殖/分化Key words
simvastatin/ dental pulp stem cells/ proliferation/ osteoblastic分类
生物科学引用本文复制引用
运慧媛,施宇光,袁梦桐..辛伐他汀对人牙髓干细胞增殖和分化的影响[J].中国美容医学,2013,22(2):263-266,4.
