中国蔬菜Issue(6)：58-62,5.
番茄温敏型核雄性不育系T-4花药蛋白质含量、POD及EST同工酶的研究
Studies on Protein Content, POD and EST Isozyme of Thermo-sensitive Genic Male-sterile Tomato T-4
摘要
Abstract
The experimental materials were thermo-sensitive genie male-sterile tomato (Lycop-ersicum esculentum Mill. ) T-4 and the control First. The protein content was determined by spec-trophotometry, adopting Coomassiebrilliant blue G250 as dye. Meanwhile, the activity of POD and EST isozyme was analyzed with the polyacrylamide gel electrophoresis of perpendicular plate. Results showed that the protein content of T-4 anther under fertile condition was significantly higher than that under sterile condition, but was no significant difference with the control First. The band of POD and EST isozyme in anther of T-4 under sterile condition was more than that in anther of T-4 under fertile condition, and the control First. There was no significant difference in band of POD and EST isozyme between T-4 and the control First, when T-4 was under fertile situation.关键词
番茄/温敏型核雄性不育系/蛋白质/POD同工酶/EST同工酶Key words
Tomato/ Thermo-sensitive genie male-sterile/ Protein/ POD isozyme/ EST isozyme分类
农业科技引用本文复制引用
国亚慧,于分弟,甘桂云,韦冠睦,王先裕,杜永臣,桝田正治..番茄温敏型核雄性不育系T-4花药蛋白质含量、POD及EST同工酶的研究[J].中国蔬菜,2013,(6):58-62,5.基金项目
国家科技部国际合作项目(2006DFA33380),广西科技厅广西科学研究与技术开发计划项目(0718007B-38),南宁市青秀区科学技术局科学研究与技术开发计划项目(20080211B),桂林市局科学研究与技术开发计划(20100112-1),现代农业产业技术体系建设专项资金(CARS-25) （2006DFA33380）
