李迎侠 1黄嘉莉 1钱新宏 1赵晓莉 1范芳 1潘凯丽1
作者信息
摘要
Abstract
Objective To study the expression of caudal-related homeodomain transcription 2 (CDX2) gene in Children with acute Ieukemia(AL) and its clinical implications, and compare with those of Wilms' tumor 1 (WT1) gene. Methods Both of the genes were detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in bone marrow (BM) and peripheral blood (PB) samples of 57 cases of children with acute leukemia-----39 cases of non-remission acute leukemia (AL) consisting of 35 cases of the newly diagnosed (25 cases of ALL, 10 cases of AML) and 4 cases of the relapsed, 18 cases of the remission. In addition, 25 cases of healthy people were enrolled as control. Results The overexpression(0.6187±0.2170)of CDX2 was found in 82.1% (32/39) AL, with the positive rate being 89.3% (25/28) in ALL and 63.6% (7/11) in AML, which showed no statistical difference (P> 0.05) and so was the expression level (0.6181 ± 0.2342 VS 0.6230 ± 0.2441) (P>0.05). In addition, CDX2 was not detectable in both the remission group and the control. Detecting samples of BM and PB from the same period of 9 newly diagnosed AL patients by semi-quantitative RT-PCR, the average CDX2 expression level was (0.4083 ± 0.2279) and (0.4124 ± 0.1749), which was of no statistical difference (P>0.05). In compariing CDX2 with WT1 in children with AL, positive rates in non-remission group (48.7% VS 82.1%) had statistical difference (P< 0.05), and those in ALL group (46.4% VS 89.3%) also showed statistically significant difference(P<0.05), while those in AML group(54.5% VS 63.6%) were of no statistical difference(P>0.05).In CDX2 positively and negatively expressed patients, positive rates of WT1 were respectively 46.8%(15/32)and 57.1 %(4/7), which suggested no statistical correlation between the expression of CDX2 and that of WT1 in AL children. In the newly diagnosed AL children, rates of complete remission (CR) in the CDX2 positively expressed patients and negatively expressed ones (96.4% VS 100%) showed no statistical difference (P> 0.05). For CDX2 and WT1, the comparison of CR rates between the double positive group and the double negative group was of no statistical difference (P > 0.05), and so were the positive group and the single positive group (P>0.05), as well as the single positive group and the double negative group (P> 0.05). Nine newly diagnosed AL patients with positive CDX2 expression were followed up for 8 to 14 months. CDX2 in 5 patients gradually turned negative during day 13 ~ 19 and the remained four turned negative after induction. However, in CDX2 negatively expressed AL patients, CDX2 remained negative at CR till present. Conclusion CDX2 gene may be more potential in children with AL and the expression level of CDX2 may have correlation with the load of leukemia, which might be of better value than WT1 in MRD detection.关键词
尾型同源盒转录因子-2/WT1基因/白血病/微小残留病/儿童Key words
Caudal-related homeodomain transcription 2/ Wilms' tumor 1/ leukemia/ minimal residual disease/ children分类
医药卫生
