中国畜牧兽医2013，Vol.40Issue(2)：31-35,5.
piggyBac转座子在哺乳动物中的应用
Application of PiggyBac Transposon in Mammals
摘要
Abstract
Since the resurrection of Sleeping Beauty (SB11), piggyBac(PB) is also described to work in mammals. DNA transposons are mobile genetic units identified in many mammals, can change the structures of genome and influence the function of special gene. The exogenous gene can be inserted into a new locus by transposase following a 'cut and paste' mechanism, so transposons have been widely used as tools to study the gene function, generate transgenic animals, and research gene therapy. Compared with SB11, PB has higher transposition level, no dose inhibition effect, and can carry larger fragments, thus PB has been cared more and more recently. Herein, we describe its mechanism of action,the factors influencing the transgenic effects,and the application in mammals.关键词
piggyBac/转座子/转基因Key words
piggyBac/ transposon/ transgene分类
生物科学引用本文复制引用
杨冠恒,曾凡一..piggyBac转座子在哺乳动物中的应用[J].中国畜牧兽医,2013,40(2):31-35,5.基金项目
国家"863"计划(2011AA020116) （2011AA020116）
国家杰出青年科学基金(81125003) （81125003）
上海市重点学科建设项目(S30201) （S30201）
国家"973"计划(2010CB945200) （2010CB945200）
卫生部国家临床重点专科建设项目. （）
