中国畜牧兽医2013，Vol.40Issue(2)：41-44,4.
鸭出血症病毒间接ELISA检测方法的建立与应用
Establishment and Application of an Indirect ELISA for Detecting DHDV
摘要
Abstract
An indirect ELISA was established to detect antibody against duck hemorrhagic disease virus (DHDV) using purified DHDV as coating antigen. The reaction conditions were optimized, including 7. 06 μg/well coating antigen of the virus, 1 : 25 dilution of testing serum and 1 : 500 dilution of HRP conjugated anti-duck IgG with cutoff-value of 0. 44 (D450nm). The specific tests showed that there were no cross-reaction to the anti-serum against duck plague virus, duck hepatitis virus, Muscovy duck parvovirus, Muscovy duck reovirus, which showed that the ELISA was specific to anti-serum against DHDV. The intro-and inter-assay indicated that the coefficient of maximum variation was 0. 0221 and 0. 0032, respectively, which showed that the method had good stability. And the result was the same as the neutralization test. The method was proved to be rapid, specific and repetitive by detecting different serum samples from immune ducks, which might be used as a serodiagnosis of DHDV.关键词
鸭出血症病毒/间接ELISA/抗体Key words
DHDV/ indirect ELISA/ antibody分类
农业科技引用本文复制引用
朱海侠,万春和,黄瑜,程龙飞,施少华,傅光华,陈红梅..鸭出血症病毒间接ELISA检测方法的建立与应用[J].中国畜牧兽医,2013,40(2):41-44,4.基金项目
现代农业产业体系建设专项资金(CARS-43) （CARS-43）
福建省种业创新与产业化工程项目(FJZYCX-9) （FJZYCX-9）
福建省农科教项目(闽财指(2007)573号) （闽财指(2007）
福建省发改委项目(闽发改投资(2008)798号文件). （闽发改投资(2008）
