中国畜牧兽医2013，Vol.40Issue(2)：53-56,4.
禽呼肠孤病毒与禽白血病病毒双重RT-PCR检测方法的建立及应用
Establishment and Application of a Double RT-PCR for ARV and ALV
马超英1
作者信息
- 1. 青海省海西州乌兰县铜普兽医站,青海海西817000
- 折叠
摘要
Abstract
To identify and differentiate rapidly the cause of clinical diseases, a double RT-PCR was developed. Two sets of specific primers were designed according to the sequences of avian reovirus (ARV) and avian leukosis virus (ALV) in the Gen-Bank. A double polymerase chain reaction was optimized to simultaneously detect two pathogens of ARV and ALV in this article. The double RT-PCR system would amplify a 485 bp fragment for ARV and a 673 bp for ALV simultaneously or separately in the samples,depending on its infection status. But not specific band amplified from other six avian pathogenic viruses. As little as 10 pg of ARV and 10 pg of ALV RNA were detected using gel electrophoresis after double RT-PCR. To evaluate the double RT-PCR, 31 clinical samples were comparatively detected. The data showed that the double RT-PCR method was 100% coincidence with the single RT-PCR, and it could be used for detection and differential diagnosis of these two viruses.关键词
双重聚合酶链反应/禽呼肠孤病毒/禽白血病病毒Key words
double RT-PCR/avian reovirus/avian leukosis virus分类
生物科学引用本文复制引用
马超英..禽呼肠孤病毒与禽白血病病毒双重RT-PCR检测方法的建立及应用[J].中国畜牧兽医,2013,40(2):53-56,4.
