中国畜牧兽医2013，Vol.40Issue(2)：80-84,5.
分段式补料批次发酵对凝结芽孢杆菌发酵水平的影响
The Influence on Fermentation Level of Bacillus coagulans by Adopting Segmented Fed-batch Fermentation Technology
摘要
Abstract
The experiment was aimed to research on fermentation level of Bacillus coagulans strain applied for animals by adopting segmented fed-batch fermentation technology. The fed-batch in exponential and stationary phases respectively increased the number of bacterium and promoted spore formation. The results showed in exponential phase that the quantity of starch additives was 10% of the total,the quantity of soya bean flour and fish meal additives was 5% of the total,the best fed-batch mode was fed twice equally (interval time for 10 to 12 h) ,on this conditions the living bacteria amount could reach 8. 30 × 109 CFU/mL from 6. 80×109 CFU/mL. The results showed in stationary phase that the best fed-batch concentration was calcium carbonate 0. 10 g/L,sodium dihydrogen phosphate 0. 156 g/L and methionine 0. 30 g/L,the best fed-batch mode was one-off feeding,on this conditions the ratio of spore-forming could reach 85. 63% from 75. 78%. Therefore, the research through the experiment on Bacillus coagulans suggested that both the living bacteria and the ratio of spore-forming of fermentation broth further increased by adopting segmented fed-batch fermentation technology.关键词
凝结芽孢杆菌/芽孢率/发酵优化/分段式补料Key words
Bacillus coagulans / the ratio of spore-forming/ fermentation optimization/ segmented fed-batch fermentation technology分类
农业科技引用本文复制引用
高书锋,孔利华,周映华,胡新旭,吴胜莲,缪东,周小玲,刘惠知..分段式补料批次发酵对凝结芽孢杆菌发酵水平的影响[J].中国畜牧兽医,2013,40(2):80-84,5.基金项目
长沙市科技攻关资金专项(K1003312-21). （K1003312-21）
