中国药房2013，Vol.24Issue(9)：828-830,3.DOI:10.6039/j.issn.1001-0408.2013.09.21
基于近红外光谱测定复方盐酸阿米洛利片有效成分的含量
Content Determination of Effective Components in Compound Amiloride Hydrochloride Tablets by Near Infrared Spectroscopy
陈久艳 1方敏 1吴再珍 1杨宏 1周群刚 1谢洪平1
作者信息
摘要
Abstract
OBJECTIVE: To establish an analytical method for the fast and simultaneous determinations of amiloride hydrochloride and hydrochlorothiazide contained in Compound amiloride hydrochloride tablet. METHODS: The multivariate calibration models of the two effective components were established using the partial least squares method between the near infrared diffuse reflectance spectra of the compound and the content of amiloride hydrochloride or hydrochlorothiazide in the compound. Based on these two models, the contents of the effective components could be calculated by using the measured spectra of the compound, and compared with the results by HPLC methods. RESULTS: For the calibration models of amiloride hydrochloride and hydrochlorothiazide , the correlation coefficients for calibration sample sets were both 0.996 2, and the correlation coefficients for prediction sample sets were 0.990 2 and 0.990 3, respectively. The root mean square errors were 0.025 2 and 0.173 5 for the calibration sample sets, and root mean square errors were 0.051 6 and 0.354 8 for the predication sample sets, respectively. Results of 2 methods were same to each other. CONCLUSIONS: Established method is simple, rapid and accurate, and it could be applied to the fast, direct and simultaneous determination for 2 components.
关键词
近红外光谱/偏最小二乘法/盐酸阿米洛利/氢氯噻嗪/含量测定Key words
Near infrared spectroscopy/Partial least squares method/ Amirolide hydrochloride/Hydrochlorothiazide/Content de-termination分类
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陈久艳,方敏,吴再珍,杨宏,周群刚,谢洪平..基于近红外光谱测定复方盐酸阿米洛利片有效成分的含量[J].中国药房,2013,24(9):828-830,3.
