中国药房2013，Vol.24Issue(9)：832-834,3.DOI:10.6039/j.issn.1001-0408.2013.09.23
HPLC法测定盐酸拉贝洛尔注射液的含量和有关物质
Determination of Content and Related Substances of Labetalol Hydrochloride Injection by HPLC
摘要
Abstract
OBJECTIVE: To establish a method to determine the content and related substances of Labetalol hydrochloride injection. METHODS: HPLC was used. The determination was carried out using Agilent TC-C18 column and methanol-0.1 mol/L sodium dihydrogen phosphate (55:45) as mobile phase at the flow rate of 1.0 ml/min. The column temperature was 50 ℃, and the detection wavelength was 230 nm. RESULTS: The resolution between the peaks of labetalol hydrochloride and free carboxylic acid and other peaks of acid degradation products was good. The linear range of labetalol hydrochloride were 41.40-372.60 (μg/ml (r= 1.000 0) with average recovery of 99.97% (RSD=0.64%, n=9). The limit of detection was 52.54 ng/ml. CONCLUSIONS: The method is simple, rapid, specific and accurate.关键词
盐酸拉贝洛尔注射液/含量测定/有关物质/高效液相色谱法Key words
Labetalol hydrochloride injection/ Content determination/ Related substances/ HPLC分类
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周怡,郑金琪..HPLC法测定盐酸拉贝洛尔注射液的含量和有关物质[J].中国药房,2013,24(9):832-834,3.
