中国医科大学学报2013，Vol.42Issue(3)：209-213,227,6.
miR-17～92基因簇在宫颈癌、子宫内膜癌与卵巢癌多种细胞系中的表达及意义
Expression and Significance of miR-17～92 Gene Clusters in Cervical Cancer, Endometrial Carcinoma and Ovarian Cancer Cell Lines
摘要
Abstract
Objective To analyze the expression of miR-17~92 gene clusters in cervical cancer,endometrial carcinoma and ovarian cancer cell lines,so as to explore the relationship between miR-17~92 gene clusters and gynecological tumors. Methods miR-17~92 gene cluster expression levels were detected using real-time polymerase chain reaction (PCR) in Hela/Siha,Ishikawa/HEC-lA,HO-8910/HO-8910 PM, SKOV3/SKOV3-TR30/SKOV3-DDP,COC1/COC1-DDP,CAOV3,and OVCAR3 cells. Target genes of miR-17~92 gene clusters and their participation in the tumor-associated signaling pathways were analyzed by miRWalk database, miR2Subpath database and KEGG pathway function clustering analysis. Results Compared with human umbilical vein endothelial cell line ECV-304,miR-17~92 gene clusters were significantly down-regulated in cervical cancer cell lines Hela and Siha (F =9.86,P < 0.001),up-regulated in endometrial cancer cell lines Ishikawa and HEC-1A(F =10.35,P < 0.005). Compared with normal ovarian cell lineIOSE386,miR-17~92 gene clusters were up-regulated in all ovarian cancer ceU lines HO-8910,HO-8910 PM.COCl ,COC1/DDP,CAOV3,OVCAR3,SKOV3,SKOV3-TR30 and SKOV3/DDP (F = 12.64,P < 0.001). Totally 30 genes including ABCA1 ,ARHGAP21 ,BAP1 ,C10orf46,CCND1,CD4,and etc. were predicted as the target of miR-17~92 gene clusters;miR-17~92 gene clusters may participated in eight biological process as suggested by miR2Subpath database enrichment analysis,and 11 signaling pathways by KEGG pathway analysis. Conclusion miR-17~92 gene clusters were widely expressed in cervical cancer,endometrial carcinoma and ovarian cancer cell lines,and the expression levels can be different in same type of tumor cells with different biological behaviors. miR-17~92 gene clusters may involve in the occurrence and development of cervical cancer,endometrial carcinoma and ovarian cancer,and influence its biological behavior through the related target genes and signaling pathways.关键词
miR-17～92基因簇/妇科肿瘤/靶基因/信号通路Key words
miR-17~92 gene cluster/ gynecological rumor/ target genes/ signal pathway分类
医药卫生引用本文复制引用
接智慧,吴建磊,陶陶,史春雪,王敏..miR-17～92基因簇在宫颈癌、子宫内膜癌与卵巢癌多种细胞系中的表达及意义[J].中国医科大学学报,2013,42(3):209-213,227,6.基金项目
国家自然科学基金资助项目(30973189) （30973189）
