中国药理学通报2013，Vol.29Issue(3)：323-327,5.DOI:10.3969/j.issn.1001-1978.2013.03.007
TGF-β/Smad3信号参与介导TRB3的促肿瘤作用
TGF-β/Smad3 signaling participates in and mediates tumor progression of TRB3
摘要
Abstract
Aim To investigate the relationship between TGF-β/Smad3 signaling and TRB3 mediated tumor progression. Methods Constitutively active Smad3 mutant ( Smad3D ) was amplified by PCR and constructed into pcDNA3. 1/Myc-His( -)B expression vector. The qualified recombinant plasmid was trans-fected into HepG2 cells with TRB3 knocking down. Then the cells were selected by G418 to establish cell line with Smad3D-Myc stably expressed. The 3TP-lux reporter activity was detected by the Dual-Luciferase Reporter Assay System. The invasion ability of cells was detected by transwell invasion assay. Results The Smad3D mutant expressing plasmid was constructed successfully. HepG2 cells with TRB3 knocking down and Smad3D overexpressing simultaneously had been established successfully. Overexpression of Smad3D increased the activity of 3TP-lux significantly, and partially reversed the inhibitory effect of tumor invasion caused by TRB3 knocking down. Conclusion TGF-β/Smad3 signaling participates in TRB3 mediated tumor invasion.关键词
TRB3/TGF-β/Smad3/Smad3D mutant/HepG2/肿瘤侵袭Key words
TRB3 / TGF-0/ Smad3 / Smad3D mutant/ HepG2/ tumor invasion分类
医药卫生引用本文复制引用
花芳,余娇娇,孙巍,胡卓伟..TGF-β/Smad3信号参与介导TRB3的促肿瘤作用[J].中国药理学通报,2013,29(3):323-327,5.基金项目
国家自然科学基金面上项目(No 30901814,81101595),中央级公益性科研院所基本科研创新药物发现与新技术专项(No 2010CHX12) （No 30901814,81101595）
