中国药理学通报2013，Vol.29Issue(3)：333-336,4.DOI:10.3969/j.issn.1001-1978.2013.03.009
瓜子金皂苷己对连二亚硫酸钠致氧糖剥夺/复供诱导PC12细胞凋亡的抑制作用
Inhibitory effect of Polygalasaponin F on the apoptosis of PC12 cells induced by sodium dithionite-caused oxygen-glucose deprivation and reperfusion
摘要
Abstract
Aim To study the effect of Polygalasapo-nin F ( PGSF ) on apoptosis of PC 12 cells induced by oxygen-glucose deprivation and reperfusion ( OGD/R ) and its mechanism. Methods The OGD/R model was established by first exposing PC 12 cells to sodium di-thionite ( Na2S2O4 ) in glucose-free Earle's solution and then by changing the culture into complete mediums, with the cell injury and apoptosis analyzed by Hoechst 33342 / PI dual staining and flow cytometry assay. The mitochondrial membrane potential ( MMP ) was determined by JC-1 staining, and the expression of Bcl-2 and Bax was detected by Western blot. Results PGSF could significantly improve the morphology of injured PC 12 cells and reduce the percentage of apop-totic cells, reverse the decrease in MMP and up-regu-late the ratio of Bcl-2/Bax proteins. Conclusion PGSF can markedly inhibit the OGD/R-induced apoptosis of PC 12 cells by regulating the expression of Bcl-2 and Bax proteins and maintaining the normal MMP.关键词
瓜子金皂苷己/连二亚硫酸钠/氧糖剥夺/复供/PC12细胞/凋亡/线粒体膜电位/Bcl-2/BaxKey words
Polygalasaponin F/ Na2S2O4/ OGD/R/ PC 12 cells/ apoptosis/ MMP/ Bcl-2/ Bax分类
医药卫生引用本文复制引用
石瑞丽,胡金凤,孔令雷,牛非,吴苗苗,何鑫,李培锋,陈乃宏..瓜子金皂苷己对连二亚硫酸钠致氧糖剥夺/复供诱导PC12细胞凋亡的抑制作用[J].中国药理学通报,2013,29(3):333-336,4.基金项目
国家自然科学基金资助项目(No U0832008,No30973887) （No U0832008,No30973887）
国家科技重大专项 ( No 2012ZX09301002-004,2012ZX09103101-006) （ No 2012ZX09301002-004,2012ZX09103101-006）
