肿瘤防治研究2013，Vol.40Issue(2)：147-150,4.DOI:10.3971/j.issn.1000-8578.2013.02.006
塞来昔布对NB4细胞增殖、凋亡及VEGF表达的影响
Effects of Celecoxib on Proliferation,Apoptosis and VEGF Expression in NB4 Cells
摘要
Abstract
Objective To explore effects of celecoxib on proliferation,apoptosis and VEGF expression in a-cute promyelocytic leukemia cell (NB4). Methods After acute promyelocytic leukemia cell line NB4 was treated by different concentrations of celecoxib, cell proliferation was detected by MTT assay. NB4 cell cycle distribution and apoptosis were measured by flow cytometry. Apoptosis related protein, the Bcl-2 expression and VEGF,HIF-la mRNA levels were detected by Western blot and RT-PCR, respectively. Results The proliferation of NB4 cells was significantly inhibited by celecoxib. Celecoxib could increase the percentage of G0/G1 cells and decrease S phase cells. The rate of apoptosis cell was increased in NB4 cells with 20 - 80 μmol/L celecoxib treatment for 24h. Celecoxib significantly induced apoptosis, and down-regulated Bcl-2 protein (P<0. 05). VEGF and HIF-la mRNA levels were inhibited in NB4 cells after celecoxib treatment. Conclusion Celecoxib could inhibit cell proliferation,arrest cycles and induce apoptosis, through down-regulation of VEGF and HIF-la in acute promyelocytic leukemia cells in vitro.关键词
急性早幼粒细胞白血病/塞来昔布/NB4细胞/细胞凋亡/细胞周期Key words
Acute promyelocytic leukemia/Celecoxib/NB4 cells/Apoptosis/Cell cycle分类
医药卫生引用本文复制引用
李杰,薛丽英,王超,王瑞仓,杨洁,郝洪岭..塞来昔布对NB4细胞增殖、凋亡及VEGF表达的影响[J].肿瘤防治研究,2013,40(2):147-150,4.基金项目
河北省卫生厅医学科学研究重点课题资助项目(20090014) （20090014）
