肿瘤防治研究2013，Vol.40Issue(3)：226-231,6.DOI:10.3971/j.issn.1000-8578.2013.03.002
自杀基因系统HSV-TK/GCV联合声动力疗法对人肺癌细胞NCI-446的体外杀伤实验
Effects of Treatment with Suicide Gene System HSV-TK/GCV Combined with Sonodynamic Therapy on Human Lung Cancer Cell NCI-446 in vitro
摘要
Abstract
Objective To observe the effects of suicide gene system HSV-TK/GCV combined with sonodynamic therapy on human lung cancer cell NCI-446 in vitro. Methods NCI-446/TK containing HSV-TK gene has been screened by the method of fluorescence and RT-PCR. MTT detected the damage effects of cells after NCI-446/TK treated with different concentrations of GCV. And bystander effect was also measured through mixed different concentrations of NCI-446 and NCI-446/TK. Meanwhile, combining HSV-TK/GCV suicide gene system and sonodynomic theragy,cell survival rate was tested by MTT,in addition,cell cycle and apoptotic rate were tested by Flow cytometry. Results With 0. 2 mg/L of GCV, the growth of NCI-446/TK was inhibited significantly (P<0. 01). With increased GCV, the inhibitory effect became obviously. When 10% NCI-446/TK in mixed cells, there was 46% total cells were inhibited. And also the inhibitory effect increased with increasing proportion of NCI-446/TK. After therapeutic alliance with suicide gene system HSV-TK/GCV and SDT,cell survival ratio was significantly lower than the groups of single treatment and the proportion of cells in G0/G1 stage, as well as apoptotic rate, were also higher than the groups of single treatment. Conclusion The lethal effect of HSV-TK/GCV suicide gene system was enhanced with increase of GCV concentration and bystander effect was also significant. When combined with SDT,the effect was better than a single treatment group.关键词
自杀基因系统/声动力疗法/肺癌细胞/联合治疗Key words
Suicide gene system/Sonodynamic therapy/ Lung cancer cells/Therapeutic alliance分类
医药卫生引用本文复制引用
李洋,马程远,里程楠,钱东华..自杀基因系统HSV-TK/GCV联合声动力疗法对人肺癌细胞NCI-446的体外杀伤实验[J].肿瘤防治研究,2013,40(3):226-231,6.
