质谱学报2013，Vol.34Issue(1)：23-28,34,7.DOI:10.7538/zpxb.2013.34.01.0023
LC-MS/MS法研究厚朴酚与和厚朴酚在大鼠体内的药动学行为
The Pharmacokinetics of Magnolol and Honokiol in Rats by LC-MS/MS
马莎莎 1邵玉凤 1吴祥猛 1王文艳 1刘万卉1
作者信息
摘要
Abstract
A rapid, sensitive and selective method for the simultaneous determination of magnolol and honokiol in plasma was described by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Magnolol and honokiol were separated on Waters XTerra C18 column using V(acetonitrile) :V(0. 1 mmol/L ammonium acetate) =75 : 25 as mobile phase by isocratic elution. The flow rate was 0. 2 mL/min, and injection volume was 5 ΜL. Deter mination was carried out by negative ion electrospray ionization mode with selected reaction monitoring. The assay was linear from 1 to 1 000 jug/L, and the lower limit of quantifica tion (LLOQ) was 1 μg/L. The limit of quantitation (S/N = 3) was 0. 1 μg/L. Intra-day and inter-day precision were less than 15%. The relative deviations were in the range of 3.4%-6.6% for magnolol and 0. 5%-4% for honokiol, respectively. The recoveries of magnolol and honokiol were more than 90%. The assay was applied to the pharmaco kinetic study of magnolol and honokiol after intragastric administration of Banxia-Houpu Decoction to rats.关键词
半夏厚朴汤/厚朴酚/和厚朴酚/液相色谱-串联质谱(LC-MS/MS)/药代动力学Key words
Banxia-Houpu decoction/ magnolol/ honokiol/ liquid chromatography-tandem mass spectrometry (LC-MS/MS) /pharmacoknietic分类
化学化工引用本文复制引用
马莎莎,邵玉凤,吴祥猛,王文艳,刘万卉..LC-MS/MS法研究厚朴酚与和厚朴酚在大鼠体内的药动学行为[J].质谱学报,2013,34(1):23-28,34,7.
