质谱学报2013，Vol.34Issue(1)：29-34,6.DOI:10.7538/zpxb.2013.34.01.0029
蛋白质胰蛋白酶水解过程双位点漏切肽段的质谱鉴定
Identification of Tryptic Peptides with Double Missed Cleavage Sites by Mass Spectrometry
摘要
Abstract
To investigate the influence of missed cleavage numbers of trypsin for database searching results, Cytochrome C and abscisic stress ripening-like protein were identified by MALDI-TOF/TOF mass spectrometry. Two and four tryptic peptides with double missed cleavage were found in the digests of cytochrome C and of abscisic stress ripening-like protein, respectively. It was found that two missed cleavage will happe when two lysine residues neighbor to each other or lysines and arginines neighbor with an aspartic acid or glu-tamic acid residue. It was observed that this phenomenon was related to histine-lysine-isole-unine/alanine sequence. In this study, the certainty of protein identification rather than protein coverage was significantly improved when tryptic peptides with double missed cleav- age sites were identified, which indicate that false negative of peptide identification will happen when the number of missed cleavage site was set to 1.关键词
胰蛋白酶/基质辅助激光解吸电离-串联飞行时间质谱/细胞色素C/大豆脱落胁迫成熟蛋白Key words
trypsin/MALDI-TOF/TOF/missed cleavage site/cytochrome C/abscisic stress ripening-like protein分类
化学化工引用本文复制引用
王勇,李水明,何曼文,邹永东..蛋白质胰蛋白酶水解过程双位点漏切肽段的质谱鉴定[J].质谱学报,2013,34(1):29-34,6.基金项目
国家自然科学基金(31070731),深圳市科技研发资金科技条件平台计划重点实验室项目(CXB201005240008A)资助 （31070731）
