中国中西医结合杂志2013，Vol.33Issue(3)：361-364,4.
五味子多糖对环磷酰胺致生精障碍大鼠的治疗作用及对生殖激素的影响
Efficacy of Schizandra Chinensis Polysaccharide on Cyclophosphamide Induced Dyszoospermia of Rats and Its Effects on Reproductive Hormones
摘要
Abstract
Objective To explore the efficacy of Schizandra Chinensis polysaccharide (SCP) on cyclophosphamide (CTX) induced dyszoospermia of rats and its effects on reproductive hormones. Methods SCP was extracted by ethanol-alkali solution. Fifty male Wistar rats were randomly divided into 5 groups,i.e., the normal control group, the model group, the low dose SCP group (100 mg/kg), the middle dose SCP group (200 mg/kg), and the high dose SCP group (400 mg/kg). Except the normal control group, the dyszoospermia rat model was established by peritoneal injection of CTX at the daily dose of 80 mg/kg, once daily for 5 successive days. After modeling, SCP was intragastrically administered at corresponding dose to the three SCP groups. Equal volume of normal saline was given to rats in the normal control group and the model group by gastrogavage. All the medication was performed once daily for 60 successive days.The blood serum and testis were withdrawal 24 h after the last intragastric administration. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) in the testis homogenate were determined by enzyme linked immunosorbent assay. The sperm count, the motility rate, and the teratospermia rate were compared. The morphology of the testis was observed using HE staining. Results Compared with the normal control group, the sperm count and the motility rate decreased, the teratospermia rate increased, the serum levels of FSH and LH increased, the T content in the testis homogenate decreased in the model group, showing statistical difference (P<0.01). Compared with the model group, the sperm count and the motility rate increased, the teratospermia rate decreased, the serum levels of FSH and LH decreased, the T content in the testis homoge-nate increased in the model group, showing statistical difference (P<0.01, P <0.05). All the indices showed dose-dependent manner in the SCP groups.The histological results showed the pathological injury in the testic-ular tissue was improved in all SCP groups.Conclusion SCP showed obvious therapeutical effects on CTX induced dyszoospermia in rats, and its mechanisms might be correlate with recovering the regulation function of hypothalamus-hypophysis-gonad axis.关键词
五味子多糖/环磷酰胺/生精障碍/生殖激素Key words
Schizandra Chinensis polysaccharide/ cyclophosphamide/ dyszoospermia/ reproductive hormone引用本文复制引用
张艳,沈楠,齐玲,陈为,董正,赵东海..五味子多糖对环磷酰胺致生精障碍大鼠的治疗作用及对生殖激素的影响[J].中国中西医结合杂志,2013,33(3):361-364,4.基金项目
吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2012]第331号) （吉教科合字[2012]第331号）
