中国中西医结合杂志2013，Vol.33Issue(3)：370-375,6.
舒郁宁心方对慢性应激抑郁模型大鼠行为学及海马BDNF、TrkB表达的影响
Effects of Shuyu Ningxin Recipe on the Praxiology and the Expressions of Hippocampal BDNF and TrkB of Model Rats with Chronic Stress-induced Depression
摘要
Abstract
Objective To observe the effects of Shuyu Ningxin Recipe (SNR) on the praxiology and the expressions of hippocampal brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase B (TrkB) of model rats with chronic stress-induced depression, thus exploring its anti-depression mechanisms. Methods Sixty adult SD rats were randomly divided into 6 groups, i.e., the normal control group, the model group, the fluoxetine group, the high dose SNR group, the medium dose SNR group, and the low dose SNR group, 10 in each group. All rats were subjected to establish chronic stress-induced depression model for 21 consecutive days. Except those in the normal control group, rats in the rest groups received gastrogavage from the 22nd day. Mice in the model group were administered with normal saline by gastrogavage. SNR at 25.0, 7.5, and 2.5 g/kg was respectively administered to rats in the high dose SNR group, the medium dose SNR group, and the low dose SNR group by gastrogavage. Fluoxetine suspension (12 mg/kg) was given to rats in the fluoxetine group by gastrogavage. All medication lasted for 3 successive weeks.The weight, open-field test, and the immobility time in forced swimming test were determined before modeling, 3 weeks (after successful modeling), and 6 weeks (by the end of medication). The expressions of hippocampal BDNF and TrkB were measured after the brain tissues were drawn by the end of the experiment. Results Compared with the normal control group, the body weight grew slowly, the behavior index decreased, the immobility time in forced swimming test was prolonged, and the expressions of BDNF and TrkB were weaken in the model group (P <0.05, P <0.01). The body weight increased, the behavior was improved, the immobility time in forced swimming test was shortened, and the expressions of BDNF and TrkB were enhanced in the high dose SNR group and the fluoxetine group by the and of medication, showing statistical difference when compared with the model group (P <0.05, P <0.01). Conclusion SNR could exert anti-depression by improving the expression levels of hippocampal BDNF and TrkB.关键词
抑郁/脑源性神经营养因子/酪氨酸激酶B/舒郁宁心方Key words
depression/ brain-derived neurotrophic factor/ tyrosine kinase B/ Shuyu Ningxin Recipe引用本文复制引用
孙志高,黄泉智,许成勇,陈利平..舒郁宁心方对慢性应激抑郁模型大鼠行为学及海马BDNF、TrkB表达的影响[J].中国中西医结合杂志,2013,33(3):370-375,6.基金项目
北京科委课题首都特色临床医学课题(No.Z090507017709030) （No.Z090507017709030）
军队十二五科研攻关项目(CWS.12J129) （CWS.12J129）
