中国中西医结合杂志2013，Vol.33Issue(3)：376-379,4.
人参皂苷Rb1对急性束缚性应激大鼠海马组织酪氨酸激酶B mRNA表达的影响
Effects of Ginsenoside Rb1 on the mRNA Expression of Tyrosine Kinase B in the Hippocampus of Acute Immobilization Stress Rats
摘要
Abstract
Objective To observe the effects of acute immobilization stress on the mRNA expression of tyrosine kinase B (TrkB) in rats' hippocampus. Methods Eighteen SD rats were randomly divided into three groups, i.e., the normal control group, the model group, and the medication group, 6 in each group. The acute immobilization stress model was prepared in the model group using acute immobilization for 2 h. Ginsenoside Rb, (40 mg/kg) was peritoneally injected to rats in the medication group 30 min before modeling, with the same procedure as those for rats in the model group. No treatment was performed to rats in the normal control group. The plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) contents were detected using ELISA. The mRNA expression of TrkB in the rats' hippocampus was detected using real-time fluorescence quantitative RT-PCR. Results Before modeling there was no statistical difference of plasma CORT or ACTH concentrations among three groups (P >0.05). The plasma CORT and ACTH concentrations increased in the model group and the medication group more significantly after modeling than before modeling, showing statistical difference (P <0.05). Besides, they were obviously higher in the model group than in the normal control group (P <0.05). They were obviously higher in the medication group than in the model control group (P <0. 05). Compared with the normal control group, the mRNA expression of TrkB significantly decreased in the model group (87.73 ±7.62 vs 50.65 ±5.19, P <0. 05), showing statistical difference. The mRNA expression of TrkB was significantly higher in the medication group (78.91 ±18.07) than in the model group, showing statistical difference (P<0.05). Conclusion Pretreatment by ginsenoside Rb1 could increase the plasma CORT and ACTH concentrations, maintain the mRNA expression of TrkB, thus relieving injury induced by acute immobilization stress.关键词
急性束缚性应激/酪氨酸激酶B/人参皂苷Rb1/促肾上腺皮质激素和皮质酮Key words
acute immobilization stress/ tyrosine kinase B/ ginsenoside Rb1/ adrenocorticotropic hormone and corticosterone引用本文复制引用
郏丹赘,张明晓,姚玉瑞,金玮,刘俊伟,王均炉,陈必成..人参皂苷Rb1对急性束缚性应激大鼠海马组织酪氨酸激酶B mRNA表达的影响[J].中国中西医结合杂志,2013,33(3):376-379,4.基金项目
浙江省自然科学基金资助项目(No.Y2101225) （No.Y2101225）
温州市科技发展项目(No.Y20090254) （No.Y20090254）
