草业学报2013，Vol.22Issue(2)：158-164,7.
20个黑麦草品系的SRAP遗传多样性分析
Genetic diversity of 20 ryegrass accessions by SRAP markers
摘要
Abstract
Sequence related amplified polymorphism (SRAP) markers were used to analyze the genetic diversity among 20 ryegrass (Lolium) lines. The results showed that 765 fragments were amplified by 53 primers. Polymorphic bands were 597 which accounted for 78. 95% in the total amplified fragments. The number of amplified and polymorphic fragments was 14. 43 and 11. 26 per primer, respectively. The genetic similarity coefficients ranged from 0. 485 to 0. 727 among these ryegrass lines and the average was 0. 627. Using unweighted pair-group method with arithmetic means (UPGMA) cluster analysis on the SRAP markers, these ryegrass lines could be divided into four groups by the threshold of 0. 63. Two Lolium multiflorum lines could not be clustered into a group but spread in 18 Lolium perenne lines. In addition, cluster results were consistent with the resources of these ryegrass lines. All results suggested that SRAP markers were suitable for the research of the genetic diversity on ryegrass.关键词
黑麦草/SRAP/遗传多样性/UPGMAKey words
ryegrass lines/ SRAP/ genetic diversity/ UPGMA分类
农业科技引用本文复制引用
李杰勤,王丽华,詹秋文,朱奎,刘井良,李万祥..20个黑麦草品系的SRAP遗传多样性分析[J].草业学报,2013,22(2):158-164,7.基金项目
安徽高等学校省级自然科学研究项目(No:KJ2011Z075),安徽科技学院青年基金(ZRC2011280),国家自然科学基金(No:31071470),安徽省"115"产业创新团队(皖人才办[2009]2号)和科技部"十二五"农村领域国家科技计划课题(No:2011BAD17B03)资助. （No:KJ2011Z075）
