草业学报2013，Vol.22Issue(2)：165-170,6.
柠条锦鸡儿CkNCED1基因启动子的克隆及表达分析
Cloning and expression analysis of the promoter of Caragana korshinskii gene
摘要
Abstract
A genomic walking technique was used to clone the promoter sequence of the CkNCEDl gene from Caragana korshinskii. The plant CARE cis-element analysis revealed that, besides the basic elements, the promoter sequence included two ABA responsive elements (ABRE) and many other stress-response elements. The PGWB533 expression vector containing this promoter sequence was constructed with the GUS reporter gene. Histochemical staining analysis showed that the GUS reporter gene was expressed in the tissues of leaves, root, stem, flower and silique, and was especially higher in leaves. These results identified the localization and expression of the CkNCED1 gene in vivo , which suggested that CkNCED1 gene plays an important role in biosynthesis regulation of ABA. The study lay a foundation for further analysis of the gene function.关键词
柠条锦鸡儿/CkNCED1基因启动子/GUS组织化学染色/ABA响应元件Key words
Caragana korshinskii/ CkNCED1 promoter/ GUS histochemical staining/ ABA responsive element分类
农业科技引用本文复制引用
任爱琴,易津,高洪文,李俊,王学敏..柠条锦鸡儿CkNCED1基因启动子的克隆及表达分析[J].草业学报,2013,22(2):165-170,6.基金项目
国家自然科学基金项目(31101755)资助. （31101755）
