大豆科学2013，Vol.32Issue(2)：211-215,5.
大豆下胚轴不定芽对卡那霉素和氯化钠耐性的研究
Kanamycin and NaCl Tolerance of Adventitious Shoot Induced from Hypocotyl in Soybean
摘要
Abstract
In order to discuss the tolerance of different genotypes of soybean varieties to kanamycin and NaCl, hypocotyl from soybean genotypes including Heinong 44, Hefeng 35 and Huaidou 9 were used as the explants to investigate the formation of adventitious shoot under stress with kanamycin and NaCl. The results showed that the tolerance to kanamycin and NaCl varied with genotypes involved. With the increasing of kanamycin and NaCl concentration, the induction rate of adventitious shoot, number of shoot and length of shoot were significantly decreased in three genotypes. The critical concentration of kanamycin for Heinong 44,Hefeng 35 and Huaidou 9 were 100,150 and 100 mg·L-1 and that of NaCl for three genotypes were 100,100 and 75 mmol·L-1 ,respectively.关键词
大豆/下胚轴/卡那霉素/NaCl/不定芽Key words
Soybean/Hypocotyl/Kanamycin/NaCl/Adventitious shoot分类
农业科技引用本文复制引用
郭秋云,王萍,刘兆普..大豆下胚轴不定芽对卡那霉素和氯化钠耐性的研究[J].大豆科学,2013,32(2):211-215,5.基金项目
国家转基因生物新品种培育重大专项(2009ZX08010-013B) （2009ZX08010-013B）
中央财政支持地方高校发展专项资金(CXTD07). （CXTD07）
