大豆科学2013，Vol.32Issue(2)：238-241,4.
两个大豆品种转hrpZpsta基因后代对大豆灰斑病的抗性分析
Analysis on Resistance to Frogeye Leaf Spot between Two Species of Transgenic Soybean Progenies with HrpZpsta Gene
摘要
Abstract
Soybean Cercospora sojina is caused by fungal pathogens,which lead to plant death. Protein harpin which coded by gene hrpZpsta has been proved to elevate resistance for plants against several kinds of fungal pathogen by hypersensitive reaction. The recombinant expressed vector which contained hrpZpsta gene had been transformed into two soybean cultivars, Jinong 17 and Jinong 29,and T4 transgenic plants were identified both in molecular way and artificial inoculations. Southern blot and RT-PCR test showed that hrpZpsta gene could inherit in T4 transgenic plants steadily on the molecular level, and artificial inoculation test revealed the resistant ability to Cercospora sojina had been improved obviously. In addition,the resistance to frogeye leaf spot varied with soybean cultivars.关键词
大豆灰斑病/hrpZpsta基因/抗病性鉴定/抗性评价Key words
Soybean Cercospora sojina/hrpZpsta gene/Screening for disease resistance/Evaluation of resistance分类
农业科技引用本文复制引用
尹俊琦,王楠,周莹,卢实,吴楠,曲静,张卓,王丕武..两个大豆品种转hrpZpsta基因后代对大豆灰斑病的抗性分析[J].大豆科学,2013,32(2):238-241,4.基金项目
转基因生物新品种培育重大专项(2011ZX08004-004). （2011ZX08004-004）
