动物营养学报2013，Vol.25Issue(4)：752-760,9.DOI:10.3969/j.issn.1006-267x.2013.04.012
饲料中维生素D3添加水平对黄鳝外周免疫相关组织细胞增殖和凋亡的影响
Effects of Vitamin D3 Supplemental Level on Cell Proliferation and Apoptosis of Peripheral Immune Related Tissues in Monopterus albus
摘要
Abstract
This experiment was conducted to study the effects of vitamin D3 supplemental level on cell proliferation and apoptosis of peripheral immune related tissues in Monopterus albus. A total of 360 healthy M. albus with an average body weight of (21. 7 ±2. 1) g were randomly assigned to 6 groups with 2 replicates per group and 30 M. albus per replicate. The M. albus in the 6 groups were fed the basal diet supplemented with 0 (control) , 250, 500, 1 000, 2 000 and 4 000 IU/kg vitamin D3, respectively. The experiment lasted for 60 days, and the hepatopancreas, head kidney, spleen and hindgut of 6 M. albus in each group were collected after feeding experiment. The proliferation and apoptosis of cells in each sample were tested by flow cytome-try. The results showed as follows-, the change trends of the cell proliferation and apoptosis of spleen, hindgut and head kidney tissues were basically identical with the vitamin D3 supplemental level increasing, which presented as the 1 000 IU/kg group had the highest cell proliferating index (PI) and the lowest cell apoptotic index (AI) , and there were significant differences between 1 000 IU/kg group and control group, 4 000 IU/kg group (P <0. 05). The PI of hepatopancreas cells was decreased with the increase of vitamin D3 supplemental level, and the lowest PI was found in 4 000 IU/kg group, in which the cell PI was significantly lower than that in the control group (P <0.05). The lowest AI of hepatopancreas cells was found in 1 000 IU/kg group, in which the cell AI was significantly lower than that in control group and 4 000 IU/kg group (P <0.05). It can be concluded that 1 000 IU/kg vitamin D3 supplementation reduces the cell apoptosis of peripheral immune related tissues, promotes the cell proliferation of head kidney, spleen and hindgut, and it is beneficial for the development of peripheral lymphoid organs in M. albus; 4 000 IU/kg of vitamin D3 supplementation hinders the cell proliferation and accelerates the cell apoptosis of peripheral immune related tissues, and induces the inhibition of immune function in M. albus关键词
黄鳝/维生素D3/细胞增殖/细胞凋亡Key words
Monopterus albus/ vitamin D3/ cell proliferation/ cell apoptosis分类
农业科技引用本文复制引用
黎德兵,李超,张龚炜,彭西,周定刚,李学伟..饲料中维生素D3添加水平对黄鳝外周免疫相关组织细胞增殖和凋亡的影响[J].动物营养学报,2013,25(4):752-760,9.基金项目
四川省教育厅自然科学重点项目(0914ZA146) （0914ZA146）
