南方医科大学学报2013，Vol.33Issue(4)：486-490,5.DOI:10.3969/j.issn.1673-4254.2013.04.05
CytoTrap酵母双杂交筛选与乙型肝炎病毒HBx蛋白相互作用的肝细胞蛋白
Screening of hepatocyte proteins interacting with hepatitis B virus x protein using CytoTrap yeast two-hybrid technique
摘要
Abstract
Objective To screen the hepatocyte proteins that interact with hepatitis B virus X protein (HBx). Methods The recombinant plasmid pSos-HBx was constructed by inserting Sos-HBx fragment into the bait vector, and after sequence verification the plasmid was transformed into competent yeast cells. The expression and self-activation of Sos-HBx protein was detected in the yeast cells. The hepatocyte proteins interacting with the bait protein was screened with CytoTrap yeast two-hybrid technique. Results The reconstructed plasmid harboring HBx gene expressed Sos-HBx protein in the yeast cells without self-activation of the protein. CytoTrap yeast two-hybrid system identified 6 hepatocyte proteins that interacted with HBx, including fibronectin 1, translationally controlled tumor protein, IQ motif and WD repeats 1, follistatin, orosomucoid 1, and disulfide isomerase family A member 3. Conclusion Six HBx-binding hepatocyte proteins have been identified using the CytoTrap yeast two-hybrid system, which provides clues for further investigation of the role of HBx protein in hepatitis and liver cancer.关键词
HBx蛋白/CytoTrap酵母双杂交/蛋白质相互作用Key words
CytoTrap yeast two-hybrid/ hepatitis B virus X protein/ protein interactions引用本文复制引用
焦佰海,文艳,刘晓佳,冯悦,张阿梅,刘丽,夏雪山..CytoTrap酵母双杂交筛选与乙型肝炎病毒HBx蛋白相互作用的肝细胞蛋白[J].南方医科大学学报,2013,33(4):486-490,5.基金项目
国家自然科学基金(30960346,81260248) （30960346,81260248）
国家科技支撑计划项目(2009BAI83B02-21,2011BAI15B01-21,2012BAI39B01) （2009BAI83B02-21,2011BAI15B01-21,2012BAI39B01）
