南方医科大学学报2013，Vol.33Issue(4)：533-537,5.DOI:10.3969/j.issn.1673-4254.2013.04.15
同型半胱氨酸调控脂肪组织PDK1的表达与糖代谢的关系
Relationship between adipose expression of 3-phosphoinositide-dependent protein kinase 1 and glycometabolism in a mouse model of hyperhomocysteinemia
摘要
Abstract
Objective To study the effect of homcysteine on the expression of 3-phosphoinositide-dependent protein kinase 1 (PDK1) in the adipose tissue and explore whether PDK1 inhibits p-Akt(Thr-308) expression and affect PI3K/Akt signal pathway to decrease glucose uptake and utilization. Methods Forty mice were randomly divided into 4 groups (n=10), namely the fasting control group, feeding control group, fasting hyperhomocysteinemia group, and feeding hyperhomocysteinemia group. In the two hyperhomocysteinemia groups, the mice were given water containing 1.5% methionine to induce hyperhomocysteinemia. Blood glucose and insulin levels in each group were determined, and the expressions of PDK1 and Akt mRNA in the adipose tissue were detected using RT-PCR; the expressions of PDK1, p-Akt(Thr-308) and Akt proteins were detected using Western blotting. Results In the fasting and feeding hyperhomocysteimia groups, blood glucose and insulin levels were significantly higher than those in the two control groups. The expressions of PDK1 mRNA and PDK1 and p-Akt (Thr-308) proteins were reduced in the two hyperhomocysteimia groups, but Akt mRNA and protein expressions were comparable with those in the control groups. Conclusion Homocysteine lowers the uptake and utilization of glucose by down-regulating PDK1 expression and affecting PI3K/Akt signal pathway to cause disturbance of glucose metabolism.关键词
高同型半胱氨酸血症/同型半胱氨酸/脂肪组织/PDK1/糖代谢Key words
hyperhomocysteinemia/ homocysteine/ adipose tissue/ 3-phosphoinositide-dependent protein kinase 1/ glycometabolism引用本文复制引用
李汝红,王雅楠,李树德,王殿华..同型半胱氨酸调控脂肪组织PDK1的表达与糖代谢的关系[J].南方医科大学学报,2013,33(4):533-537,5.基金项目
国家自然科学基金-云南联合基金项目(U1132606) （U1132606）
云南省重大攻关项目(2009CA010) （2009CA010）
云南省科技厅-昆明医科大学联合专项基金项目(2009CD213,2012FB101) （2009CD213,2012FB101）
