福建农林大学学报（自然科学版）2013，Vol.42Issue(2)：158-161,4.
孑遗植物水松基因组DNA提取及ISSR反应体系建立
Extraction of genomic DNA and optimization of ISSR reaction system with orthogonal design in Glyptostrobus pensilis
摘要
Abstract
The improved CTAB method was used for the extraction of genomic DNA in different populations of Glyptostrobus pensilis, and a satisfactory ISSR reaction system was also established by orthogonal design. The result showed that the improved CTAB method was suitable for the extraction of DNA, and the DNA extracted by this method had higher yield, better quality, and could be used for ISSR-PCR reaction. A number of factors, which affected the results of ISSR - PCR reaction, such as quantity of template DNA, primer, concentration of dNPT and Taq DNA polymerase were studied.关键词
水松/DNA提取/ISSR-PCR/体系优化Key words
Glyptostrobus pensilis/ extraction of DNA/ ISSR analysis/ system optimization分类
农业科技引用本文复制引用
郑世群,刘金福,吴则焰,洪伟,徐道炜,何中声..孑遗植物水松基因组DNA提取及ISSR反应体系建立[J].福建农林大学学报（自然科学版）,2013,42(2):158-161,4.基金项目
福建省自然科学基金(2009J01053) （2009J01053）
福建省科技厅资助高校项目(2006F50009). （2006F50009）
