福建农林大学学报（自然科学版）2013，Vol.42Issue(2)：166-170,5.
红麻叶绿体DNA非编码区扩增及PCR-RFLP多态性分析
The non-coding regions sequence characteristics of chloroplast DNA in kenaf and analysis on PCR-RFLP polymorphism
摘要
Abstract
To study the chloroplast DNA (cpDNA) non-coding regions sequence characteristics in kenaf, with the genomic DNA of eight kenaf photoperiod-insensitive mutants and their four related species as template, we amplified cpDNA non-coding regions in kenaf using eight cpDNA universal primer pairs. Firstly, we optimized the PCR amplifing conditions of cpDNA non-coding sequence, then digested the products by four restriction enzymes (EcoR I , HindW , Taq I , Aid I ). The results showed that the u-niversal primers were suitable for the kenaf cpDNA non-coding sequence amplifing, the products were detected by 1.5% agarose gel electrophoresis, which showed fragment size ranged from 400 to 2000 bp, the total fragments size was about 13000 bp. The digested PCR amplification products had the same digesting site, but only the product amplified by primers cp4 had polymorphic fragments after being digested by Alu I , which suggested the cpDNA sequence between kenaf photoperiod-insensitive mutants and controlled varieties existed variation. Therefore, the above results could provide a theoretical basis on further exploring the relationship of chloroplast genome variation between intraspecific kenaf.关键词
红麻/叶绿体DNA/PCR-RFLP/光钝感Key words
kenaf/ chloroplast DNA/ PCR-RFLP/ photoperiod-insensitive分类
农业科技引用本文复制引用
徐建堂,祁建民,林培清,张高阳,陶爱芬,方平平..红麻叶绿体DNA非编码区扩增及PCR-RFLP多态性分析[J].福建农林大学学报（自然科学版）,2013,42(2):166-170,5.基金项目
农业部东南黄/红麻科学观测实验站项目(农科教2011-9) （农科教2011-9）
福建省自然科学基金资助项目(2011J05046). （2011J05046）
