广西医科大学学报2013，Vol.30Issue(1)：5-7,3.
糖皮质激素性骨质疏松大鼠模型建立的研究
ESTABLISHMENT OF GLUCOCORTICOID-INDUCED OSTEOPOROSIS RAT MODEL
摘要
Abstract
Objective: To analyze the establishment of glucocorticoid-induced osteoporosis (GIOP) animal model. Methods: Forty Sprague Dawley (SD) rats of 3. 5-month-old were recruited, which were randomly assigned to the model or control groups, each group included 20 rats (one half male, the other half female). Model group was injected dexamethasone 2. 5 mg/kg intramuscularly, twice a week, while the control group was injected equivalent normal saline intramuscularly. Bone mineral density (BMD) of the whole body in vivo and the femur in vitro was measured by dual energy X-ray absorptiometry at the end of the ninth week. Results: The mortality was higher in the model group than in the control group (40% vs. 10% , P <0. 05). The mortality of female and male rats was 20% (2/10) and 60% (6/10) in model group (female/male = 1 : 3), respectively. BMD was significantly lower in the model group than in the control group ( P <0. 05). The changes of BMD of the whole body in vivo and the femur in vitro had a good agreement. Trabecula of the model group was sparse, fracture, arrangement disorder under microscope. Conclusion: GIOP animal model can be successfully established by injecting dexamethasone 2. 5 mg/kg intramuscularly, twice a week for 9 weeks. BMD of whole body in vivo and femur in vitro can be used to evaluate the GIOP animal model.关键词
骨质疏松/糖皮质激素/大鼠模型Key words
osteoporosis/ glucocorticoid/ rat model分类
医药卫生引用本文复制引用
田丽花,梁敏,张劼,冼晶,黄松,蔡劲薇,罗佐杰..糖皮质激素性骨质疏松大鼠模型建立的研究[J].广西医科大学学报,2013,30(1):5-7,3.基金项目
国家自然科学基金资助项目(No.81260142) （No.81260142）
