广西医科大学学报2013，Vol.30Issue(1)：69-73,5.
大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响
EFFECT OF ALLICIN COMBINED WITH VINCRISTINE ON PROLIFERATION AND APOPTOSIS IN BLADDER CANCER CELL LINE BIU-87
摘要
Abstract
Objective:To investigate the effect of allicin combined with vincristine on proliferation and apop-tosis in bladder cancer cell line BIU-87. Methods: BIU-87 cells with different treatments were divided into allicin, vincristine, and combination groups, and a non-drug treatment group was the blank control group. Inhibitory effect of allicin and vincristine on bladder cancer cell line BIU-87 proliferation was assessed by CCK-8 assay. The mRNA and protein levels of CDK1 and CyclinBl in BIU-87 cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry and Western-blot. Cell apoptosis was analyzed by flow cytometry (FCM). Results: Anti-proliferation rate of bladder cancer cell line BIU-87 by allicin and vincristine treated is evident, in a dose-dependent manner. The combination group evidently reduced the expression of CDK1 and CyclinBl mRNA and protein in the BIU-87 cells, as compared with the blank control group ( P <0. 05). Both allicin and vincristine can also induce apoptosis. The apoptosis rate of combination group [(54. 89 ± 0. 27)%] was statistical significance ( P <0. 05) as compared with the blank control group [(3. 81±0. 63)%], the allicin group [(19. 20 ± 0. 26)%], and the vincristine group [(25. 39 ± 0. 31)%]; respectively. Conclusion: Allicin can enhance vincristine on the proliferation inhibition in bladder cancer cell line BIU-87, both of which have the synergistic antitumor effects; the mechanism of apoptosis induced by allicin may be associated with the down-regulation of the expression of CDK1 and CyclinBl.关键词
膀胱癌/大蒜素/长春新碱/CDK1/CyclinB1Key words
bladder cancer/ allicin/ vincristine/ CDK1 / CyclinBl分类
医药卫生引用本文复制引用
李永生,王允金,穆细院..大蒜素联合长春新碱对膀胱癌BIU-87细胞增殖和凋亡的影响[J].广西医科大学学报,2013,30(1):69-73,5.基金项目
福建省科技计划资助项目(No.2007F3036) （No.2007F3036）
