工业微生物2013，Vol.43Issue(2)：33-37,5.DOI:10.3969/j.issn.1001-6678.2013.02.007
一株兼产淀粉酶和蛋白酶的烟碱降解菌的分离与鉴定
Screening, isolation and identification of a high efficient nicotine-degrading strain with protease and amylase activities
摘要
Abstract
32 nicotine-degrading bacterial strains were separated and screened from tobacco and soil with selectivity culture medium, in which nicotine was the sole source of carbon and nitrogen. Among them, strain A3 was the best one with strong ability to degrade nicotine, and it could secrete protease and amylase simultaneously. This strain was inoculated in the medium with 0.1% nicotine as carbon and nitrogen source, and incubated at 30℃ for 18 h with reciprocal shaking (200 r/min) ,the ratio of nicotine degradation was 96. 3% ; and the ratio of degradation was 36.1% when the strain was inoculated in 0.2% nicotine for 48 h, meanwhile, the activities of protease and amylase were 42.6 U/mL and 16.5 U/mL respectively. According to the normal morphology, physiological, biochemical characteristics and 16S rDNA gene sequence, the strain A3 was identified as Arthrobacter rdcotianae GYC103.关键词
烟碱/烟草节杆菌/蛋白酶/淀粉酶Key words
nicotine/ Arthrobacter nicotianae/ protease/ amylase引用本文复制引用
龙章德,韦方立,黄泰松,韦建玉,白森,胡亚杰,蔡联合,申佩弘..一株兼产淀粉酶和蛋白酶的烟碱降解菌的分离与鉴定[J].工业微生物,2013,43(2):33-37,5.基金项目
广西中烟工业有限责任公司创新项目(20100037). （20100037）
