甘蔗糖业Issue(1)：38-42,5.
聚乙二醇-硫酸铵双水相体系萃取α-葡聚糖酶
Extraction of Dextranase by PEG/(NH4)2SO4 Aqueous Two-Phase Systems
黎志德 1蚁细苗 1黄思鸿 1曾练强 1黄曾慰 1常国炜 1柳颖 1梁达奉1
作者信息
摘要
Abstract
In the PEG(polyethylene glycol)(NH4)2 aqueous two-phase system, the effects of the PEG molecular weight, PEG and ammonium sulfate concentration on the dextranase extraction were studied. According to parameters of dextranase activity, protein concentration, specific activity and extraction rate, we can find the best technological parameter. The results indicated that the best PEG molecular weight was 4000, the best PEG4000 concentration was 13%, and the best ammonium sulfate concentration was 13%. In this condition, specific activity of end product is 2364.97 U/mg and extraction rate is 99.94%.关键词
聚乙二醇/双水相萃取/α-葡聚糖酶Key words
Polyethylene glycol/ PEG/ Two-phase systems/ Dextranase分类
轻工纺织引用本文复制引用
黎志德,蚁细苗,黄思鸿,曾练强,黄曾慰,常国炜,柳颖,梁达奉..聚乙二醇-硫酸铵双水相体系萃取α-葡聚糖酶[J].甘蔗糖业,2013,(1):38-42,5.
