郑州大学学报（医学版）2013，Vol.48Issue(2)：175-178,4.DOI:10.3969/j.issn.1671-6825.2013.02.008
银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Caspase-3及VEGF mRNA表达的影响
Effects of ginkgolide B on expression of Caspase-3 and VEGF mRNA in brain tissue of newborn rats with hypoxic-ischemic brain damage
摘要
Abstract
Aim:To observe the effect of ginkgolide B ( GB ) on mRNA expression of Caspase-3 and vascular endothelial growth factor ( VEGF ) in brain tissue of hypoxic-ischemic brain damage ( HIBD ) newborn rats. Methods:A total of 96 clean 7-day-old health SD rats were randomly divided into sham operation group, the model group, low and high dose GB treatment groups. Classic Rice method was used to establish HIBD model in the latter 3 groups. 4 h after operation, GB at dose of 5 mg/kg and 10 mg/kg was given to rats in the low and high dose GB treatment groups by intraperitoneal injection postoperation, once a day for 5 days, sham operation and model groups were given equal physiological saline. 6 rats in each group were sacrificed at the 3rd,7th, 14th and 28th day after model establishment,respectively. Quantitative real-time fluorescent PCR was employed to detect the expressions of Caspase-3 and VEGF mRNA in brain tissue. Results:The expressions of Caspase-3 and VEGF mRNA had no significant differences among 4 time points in sham operation group( F = 0. 134,0. 382,P >0.05 ),but in the model group, low and high dose GB treatment groups, the expressions of Caspase-3 and VEGF mRNA reached the peak at the 3rd day,then decreased, and reached the level of the sham operation group at the 28th day after operation( F = 109. 356,33. 281 ,9. 703 and 39. 945 ,103. 903 ,60. 956,P <0. 001 ). At the 3rd,7th, 14th day after operation, the expressions of Caspase-3 and VEGF mRNA had significant differences among the 4 groups( F = 128.865 and 97. 912 ,40.058 and 57.121,24. 184 and 8. 621 ,P <0. 001 );compared with sham operation group, the expressions of Caspase-3 and VEGF mRNA in model group were higher,while the expression of Caspase-3 mRNA was lower and that of VEGF mRNA was higher in low and high dose GB treatment groups when compared with the model group( P < 0. 05 ), and the changes of high dose GB treatment group was more significant( P < 0. 05 ). Conclusion:GB can repair brain damage after HIBD through down-regulating Caspase-3 expression and up-regulating VEGF expression.关键词
缺氧缺血性脑损伤/新生/大鼠/银杏内酯B/Caspase-3/血管内皮生长因子Key words
hypoxic-ischemic brain damage/newborn/rat/ginkgolide B/Caspase-3/vascular endothelial growth factor分类
医药卫生引用本文复制引用
王军,尚凤伟,朱登纳,王宝珍,张冬秀,张贞焕,马洁琼..银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Caspase-3及VEGF mRNA表达的影响[J].郑州大学学报（医学版）,2013,48(2):175-178,4.基金项目
河南省教育厅自然科学研究项目 2009A320039 （）
