郑州大学学报（医学版）2013，Vol.48Issue(2)：188-193,6.DOI:10.3969/j.issn.1671-6825.2013.02.012
3R4F参比卷烟主流烟气总粒相物的Ames试验
Ames test for total particulate matter of 3R4F reference cigarette mainstream smoke
摘要
Abstract
Aim:To study the mutagenicity of total particulate matter( TPM ) of 3R4F reference cigarette mainstream smoke examined with Ames test. Methods:Using 4 strains Salmonella Typhimurium TA97 , TA98 , TA100 and TA102 , mutagenic capacity of reference cigarette 3R4F was detected; the sensitive strains were screened as experimental subject, and volume fraction 5% and 10% S9 mixtures were compared for optimal activity and different mutagenic effects were analyzed with different pre-incubation time. Results:TA98 and TA100 were more sensitive than TA97 and TA102 in Ames test with S9 mixture. The active effect of 5% S9 was similar with 10% S9. Mutagenicity of TPM was the most effective using 20 minute pre-incubation at 37℃ . Conclusions:By using this modification of Ames assay with pre-incubation in TA98 and TA100 and 5% S9 mixture, the mutagenic effects of TPM of cigarette smoke could be effectively detected.关键词
卷烟主流烟气/总粒相物/Ames试验/致突变Key words
cigarette mainstream smoke/total particulate matter/Ames assay/mutagenicity分类
医药卫生引用本文复制引用
尚平平,李翔,聂聪,赵乐,孙学辉,彭斌..3R4F参比卷烟主流烟气总粒相物的Ames试验[J].郑州大学学报（医学版）,2013,48(2):188-193,6.基金项目
国家科技支撑计划基金资助项目 2012BAK01B03 （）
