郑州大学学报（医学版）2013，Vol.48Issue(2)：210-213,4.DOI:10.3969/j.issn.1671-6825.2013.02.018
THP-1细胞系白血病干细胞的检测、分选及鉴定
Sorting and identification of leukemia stem cells in THP-1 cell line
王颖超 1冯磊 1殷楚云 1魏永纬 1马丽娜 1王春美 1盛光耀1
作者信息
摘要
Abstract
Aim:To explore whether the human acute monocytic leukemia THP-1 cell line contains leukemia stem
cells ( LSCs ) or not,and to compare the biological properties between LSCs and non-LSCs. Methods:Flow cytometry was used to detect the percentage of LSCs ( CD34+ CD38- cell ) in THP-1 , and then the cell cycle of LSCs and non-LSCs was compared. The mRNA expressions of multidrug resistance proteins ( ABCG2, ABCB1 ) and apoptosis gene ( Bcl-2, Bax ) were examined by real time quantitative PCR in two subpopulations. NOD/SCID mice were injected with different subpopu-lations of THP-1 ,and the incidence of leukemia was compared. Results:LSCs almost accounted for ( 0.12 ±0. 06 )% of viable cells in THP-1, most of which were in rest stage, and the G0/G1 phase cells accounted for ( 94. 03 ± 1. 61 )% . While the S/G2/M phase cells in non-LSCs accounted for ( 56. 58 ± 1. 59 )% . The mRNA expressions of ABCG2, ABCB1 and Bcl-2 in LSCs were higher than those in the non-LSCs ( t = 20.725 ,22. 179 ,13. 707 ,P <0. 001 ). Although there were no differences between these two subpopulations in Bax expression( t = 1. 635 ,P = 0. 071 ); Bcl-2/Bax value in LSCs was significantly higher than that in the non-LSCs ( t = 10. 781 ,P < 0.001 ). The systemic disseminated leukemia model was established successfully by injecting 1 × 103 LSCs in 4 weeks after injection. Non-LSCs requires at least 1 × 106 to establish tumor model. Conclusions:The THP-1 cell line contains LSCs, and the proportion of LSCs is low. Most LSCs stay quiescent and have biological property of stem cells as well as strong tumorigenic property, and they may be associated with tumor resistance and recurrence.关键词
THP-1细胞/白血病干细胞/ABC转运体/NOD/SCID小鼠Key words
THP-1 cell/leukemia stem cell/ATP-binding cassette transport/NOD/SCID mouse分类
医药卫生引用本文复制引用
王颖超,冯磊,殷楚云,魏永纬,马丽娜,王春美,盛光耀..THP-1细胞系白血病干细胞的检测、分选及鉴定[J].郑州大学学报（医学版）,2013,48(2):210-213,4.
