扬州大学学报（农业与生命科学版）2013，Vol.34Issue(1)：23-27,5.
不同日龄鸭肝脏中IGF-1及IGF-1R基因表达规律的研究
Study on the patterns of IGF-1 and IGF-1R gene expression in liver tissue of duck
摘要
Abstract
This study was to find out the developmental patterns of insulin-like growth factor-1 (.IGF-1) and IGF-1 receptor (IGF-1R) gene expression in different duck breeds in liver tissue. Real time PCR was applied to investigate and compare the developmental patterns of IGF-1 and IGF-1R gene in liver tissue of duck. 10 of Gaoyou duck and Jinding duck were sampled at the age of 0, 14, 28, 42, 56 day respectively. The results showed that the expression levels of IGF-1 mRNA in liver tissue in Gaoyou duck and Jinding duck increased first and declined thereafter gradually during 0 to 56 day. The expression of IGF-1 gene in Gaoyou duck had very significant difference with that in Jinding duck at the age of 56 day (P<0. 01). The expression levels of IGF-1R mRNA in liver tissue declined gradually from the age of 0 to 56 day. The expression of IGF- 1R gene in Gaoyou duck at the age of 0 day was significantly higher than that in Jinding duck. The developmental patterns of IGF-1 and IGF-1R gene expression in Gaoyou duck and Jinding duck had negative correlation in various degrees with body weight. The IGF-1 and IGF- 1R mRNA expression in liver tissue of duck followed specific developmental patterns respectively. Level of IGF-1R mRNA expression did not depend on that of IGF-1 mRNA expression.关键词
鸭/肝脏/IGF-1基因/IGF-1R基因/实时荧光定量PCRKey words
duck/ liver tissue/ IGF-1 gene/ IGF-1R gene/ real time polymerase chain reaction分类
农业科技引用本文复制引用
常明雪,朱文奇,章玲玲,宋卫涛,宋迟,胡艳..不同日龄鸭肝脏中IGF-1及IGF-1R基因表达规律的研究[J].扬州大学学报（农业与生命科学版）,2013,34(1):23-27,5.基金项目
国家自然科学基金资助项目(31172194) （31172194）
江苏省科技支撑计划项目(BE2011329) （BE2011329）
现代农业品种创新计划项目[CX(11)1030] （11）
江苏省家禽科学研究所青年基金资助项目(JQ201103) （JQ201103）
