扬州大学学报（农业与生命科学版）2013，Vol.34Issue(1)：36-40,5.
扬州市近3年新甲型H1N1流感病毒HA基因分析
Genetic analysis of HA genes of new influenza A (H1N1) viruses isolated in Yangzhou area during nearly 3 years
摘要
Abstract
Respiratory tract swabs were collected and screened by a Real-time PCR method using a new influenza A (HIND virus detection kit. The positive samples were inoculated into MDCK cells for virus isolation. The HA genes of influenza A viruses were amplified by RT-PCR and were subject to sequence. The genetic characteristic of HA genes of new influenza A (HIND viruses isolated in Yangzhou area between 2009 and 2011 was analysed. The results showed that 9 new influenza A (HIND viruses were isolated. Their nucleotide and amino acid homologies of HA genes among 9 isolates were 98. 1% —99. 8% and 96. 6%-99. 8%, respectively. Compared with vaccine strain A/California/07/2009, the nucleotide and amino acid homologies of HA genes were 98. 4% — 99.5% and 97. 0% — 99. 3% , respectively. There were point mutations in antigenic epitope of HA protein of three viruses isolated in 2011. Polygenetic analysis revealed that there were three sublineage derived from 9 isolates. These data indicate that antigenic drift of HA protein has occurred among these new influenza A (HIND virus isolates.关键词
新甲型流感病毒/H1N1/血凝素/遗传进化分析Key words
New influenza A virus/ H1N1/ hemagglutinin/ polygenetic analysis分类
农业科技引用本文复制引用
肖越胜,宿春虎,黄瑶,张军,彭大新..扬州市近3年新甲型H1N1流感病毒HA基因分析[J].扬州大学学报（农业与生命科学版）,2013,34(1):36-40,5.基金项目
国家重点基础研究发展计划项目(973-2011CB505003) （973-2011CB505003）
