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不同处理技术对环境扫描电镜下细菌原始形态的影响

孙镇平 李佳 刘洪红 王忠敏 孙伟 李敬盼 单哲 薛太平 顾玉泉

扬州大学学报(农业与生命科学版)2013,Vol.34Issue(1):41-43,3.
扬州大学学报(农业与生命科学版)2013,Vol.34Issue(1):41-43,3.

不同处理技术对环境扫描电镜下细菌原始形态的影响

The impact of different processing techniques on the original form of environmental scanning electron microscope bacteria

孙镇平 1李佳 1刘洪红 1王忠敏 1孙伟 1李敬盼 1单哲 1薛太平 1顾玉泉1

作者信息

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摘要

Abstract

The application of conventional high vacuum scanning electron microscopy of biological sample must be dried and treated conductively, but for both of the freeze drying or critical point drying, there exist problems that the samples have different degrees dehydration distortion. A lot of biological materials such as fungal hyphae and spores, aquatic algae, culture cell, insects, embryo and larvae processed can produce large deformation, and the original appearance of organisms can not be observed. This study improved the bacterial samples through fixed preparation technology, and the bacteria samples of natural original form can be observed by the environmental scanning electron microscope (ESEM).

关键词

环境扫描电镜/生物样品/原始形态/技术改进

Key words

environmental scanning electron microscopy/ biological samples/ primitive form/ technical improvements

分类

生物科学

引用本文复制引用

孙镇平,李佳,刘洪红,王忠敏,孙伟,李敬盼,单哲,薛太平,顾玉泉..不同处理技术对环境扫描电镜下细菌原始形态的影响[J].扬州大学学报(农业与生命科学版),2013,34(1):41-43,3.

基金项目

江苏省科技厅前瞻性高新技术研究项目(BY2011138) (BY2011138)

江苏高校优势学科建设工程资助项目 ()

扬州大学学报(农业与生命科学版)

OA北大核心CSCDCSTPCD

1671-4652

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